This learning resource focuses on biosecurity protocols and the proper use of personal protective equipment (PPE) when handling animal specimens in a laboratory setting. The objectives include learning the importance of biosecurity, identifying the three critical PPE items necessary for safe handling (gloves, goggles, and apron), and understanding the correct sequence for removing PPE to prevent cross-contamination between specimens. The resource outlines a scenario-based doffing sequence, emphasizing why each step is crucial for safety in multi-species labs. Additionally, it includes a self-assessment against the Standard Operating Procedure (SOP) for doffing PPE, and encourages reflective analysis on the importance of adhering to biosecurity measures to mitigate risks of zoonotic disease transmission.