This learning resource explores the differences between two microscopic organisms: E. coli bacteria and Amoeba proteus. The focus is on observing and identifying the distinct features found at the centers of these organisms. The activity encourages students to closely examine the unique characteristics of each organism, fostering skills in observation and comparison. Participants will learn how to differentiate between the two by analyzing visual cues, specifically noting what is present in the center of Amoeba proteus that is absent in E. coli.